Coding
Sticky fli

Part:BBa_K2845000:Design

Designed by: Oliver Baltensperger, Antonio Martínez Brotons   Group: iGEM18_ETH_Zurich   (2018-10-09)


Flagellar protein FliC containing a mutation, which makes it sticky.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 736
    Illegal BamHI site found at 1071
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
    Illegal AgeI site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FliC (a.k.a. FlaA or flagellin) is the protein, which builds up the filament of the bacterial flagellum. By introducing the mutation, we created the truncated version “sticky fliCâ€, which still expresses a fully functional flagellar protein. The bacteria expressing only sticky fliC will still build flagella and maintain their motility. With this mutation the flagella have a higher tendency to adhere to surfaces like glass or polystyrene. We utilized this feature to specifically tether beads to the flagellum, which results in spinning beads, due to the natural rotation of the flagellum.